Molecular Biology (S1 Lab)

Practicals for the Bio and Process Engineering and Technical Medicine programmes take place in the Molecular Biology Lab. In addition students carry out practical coursework and thesis work and research practicals. The lab is equipped for genetic engineering experiments at security level 1.

  • Cloning and characterization of recombinant proteins
  • Restriction, ligation and transformation (various methods)
  • CRISPR-Cas technology
  • Gene expression experiments
  • (PCR-based) characterization of recombinant micro-organisms
  • Genotyping

Equipment:

  • Incubators for the cultivation of (recombinant) micro-organisms
  • Electroporator
  • PCR thermoblocks
  • Real-time PCR cycler (LightCycler, Roche)
  • Agarose gel electrophorese module with gel documentation system
  • SDS-PAGE and Western Blot module for evidence of expression of (recombinant) proteins
  • Cold store, -20 and -80°C storage systems
  • Sterile workstation
  • Autoclave

Examples of student experiments in this lab:

  • Cloning and characterization of recombinant proteins
  • CRISPR Cas for gene knockout in E. coli
  • Genotyping of apples from apple orchards
  • Expression of recombinant Taq DNA polymerase

Room

A 2.08b -2.10 Villingen-Schwenningen